Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Liver

Cell type

SNU-475

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

sample_name

SNU475_4_Dox_minus_CTCF_r2

antibody

CTCF (Cell Signaling Technology, #3418S, Lot 4)

biological_replicate

2

biomaterial_provider

American Type Culture Collection (ATCC)

cell_line

SNU475

cell_type

hepatocellular carcinoma

culture_collection

ATCC: CRL-2236

dev_stage

adult

disease

hepatocellular carcinoma

genetic_modification

Lentiviral/Retroviral vector-mediated

growth_condition

RPMI-1640 with 10% fetal bovine serum (FBS)

growth_protocol

maintained as adherent cells

sample_type

cultured cells

sex

male

temp

37

Sequenced DNA Library

library_name

475_4_m_CTCF_r2

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

"QIAseq Ultralow Input Library Kit (QIAGEN, #180492)"

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

59939017

Reads aligned (%)

98.1

Duplicates removed (%)

20.4

Number of peaks

1454 (qval < 1E-05)

hg19

Number of total reads

59939017

Reads aligned (%)

97.2

Duplicates removed (%)

21.1

Number of peaks

1037 (qval < 1E-05)